Human IgM (Mu chain specific) Goat Polyclonal Antibody

CAT#: R1340AP

Human IgM (Mu chain specific) goat polyclonal antibody, AP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other AP-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended dilutions
  ELISA: 1/1,000-1/3,000.
  Western blot: 1/500-1/2,000.
  Immunohistochemistry: 1/200-1/1,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgM μ mu chain
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Human IgM and Human Serum.
  No reaction was observed against Human IgG F(c).
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other human heavy or light chain isotypes.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) sodium azide.
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: AP
• Storage: Store the antibody (undiluted) at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Dilute only prior to immediate use.