Human IgA + IgG + IgM Goat Polyclonal Antibody

CAT#: R1343AP

Human IgA + IgG + IgM goat polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for immunoblotting (western blot: 1/500-1/3,000, dot blot), ELISA (1/2,000-1/10,000) and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG, IgA and IgM whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum.
  This reagent is suitable for the detection of all human immunglobulin isotypes, subclasses and chain combinations.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) and 0.01% (w/v) Sodium Azide
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody (undiluted) at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial
  loss of enzymatic activity.
  Dilute only prior to immediate use.