Human IgA + IgG + IgM Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommended Dilution | Western Blot: 1/5000-1/20000. ELISA: 1/400000. Immunohistochemistry on frozen and paraffin sections: 1/1000-1/5000. |
Reactivities | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Human IgG, IgA and IgM whole molecules |
Specificity | This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Goat Serum. This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Label: Biotin State: Lyophilized purified IgG fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% (w/v) Sodium Azide Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule. |
Reconstitution Method | Restore with 1.0 ml deionized water or equivalent. |
Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography |
Conjugation | Biotin |
Storage | Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
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