Human IgA + IgG + IgM Goat Polyclonal Antibody

CAT#: R1343HRP

Human IgA + IgG + IgM goat polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/20,000-1/100,000.
  Western blot: 1/2,000-1/10,000.
  Immunoprecipitation: 1/1,000-1/5,000.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG, IgA and IgM whole molecules in goat.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum, Human IgG, Human IgA and Human IgM.
  This reagent is suitable for the detection of all Human immunoglobulin isotypes and chain combinations.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.