Monkey IgG (gamma chain specific) Goat Polyclonal Antibody

CAT#: R1344AP

Monkey IgG (gamma chain specific) goat polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western Blot: 1/500-1/2,500, Dot blot), ELISA (1/500-1/8,000) and Immunohistochemistry (1/200-1/1,000) as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Monkey IgG γ (gamma chain).
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Monkey IgG and Monkey Serum.
  No reaction was observed against Monkey IgA or Monkey IgM.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other monkey heavy or light chain isotypes.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
  Label: AP
  State: Liquid (sterile filtered) purified IgG fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody (undiluted) at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial
  loss of enzymatic activity.
  Dilute only prior to immediate use.