Monkey IgG (gamma chain specific) Goat Polyclonal Antibody

CAT#: R1344F

Monkey IgG (gamma chain specific) goat polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  FLISA: 1:10,000-1:50,000
  Immunofluorescence: 1:1,000-1:5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Monkey IgG g gamma chain.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Monkey IgG and Monkey Serum.
  No reaction was observed against Monkey IgA or Monkey IgM.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other Monkey heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 5.0 moles FITC per mole of Goat IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: FITC
• Storage: Store the antibody at 2-8°C, reconstitute and store at 2-8°C for one month or at -20°C for longer. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.