Monkey IgM (Mu chain specific) Goat Polyclonal Antibody

CAT#: R1345B

Monkey IgM (Mu chain specific) goat polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/20,000-1/300,000.
  Western Blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
  This product has been assayed against 1.0 ug of Monkey IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/5,000 to 1/20,000 of the concentration is suggested for this product.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Monkey IgM µ chain
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Monkey IgM and Monkey Serum.
  No reaction was observed against Monkey IgA or Monkey IgG.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (Ig and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: Biotin (Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC))
  Molar radio: 10-20 BAC molecules per Goat IgG molecule.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  Avoid repeated freezing and thawing.