Monkey IgA (alpha chain specific) Goat Polyclonal Antibody
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Specifications
Product Data | |
Recommended Dilution | Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: ELISA: 1/100,000. Western blot: 1/2,000-1/5,000. Immunohistochemistry: 1/1,000-1/5,000. |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Monkey IgA alpha chain. |
Specificity | Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Monkey IgA and Monkey serum. Specificity was confirmed by ELISA at less than 5% cross reactivity against Monkey IgM or Monkey IgG. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative. Label: Biotin State: Lyophilized purified Ig fraction. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Molar radio: 10-20 BAC molecules per Goat IgG molecule |
Reconstitution Method | Restore with 1.0 ml deionized water (or equivalent). |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity Chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. |
Conjugation | Biotin |
Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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