Monkey IgA (alpha chain specific) Goat Polyclonal Antibody

CAT#: R1346B

Monkey IgA (alpha chain specific) goat polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/100,000.
  Western blot: 1/2,000-1/5,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Monkey IgA alpha chain.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Monkey IgA and Monkey serum.
  Specificity was confirmed by ELISA at less than 5% cross reactivity against Monkey IgM or Monkey IgG.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Biotin
  State: Lyophilized purified Ig fraction.
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Goat IgG molecule
• Reconstitution Method: Restore with 1.0 ml deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Monkey IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.