Mouse IgG (H+L chain), adsorbed Donkey Polyclonal Antibody

CAT#: R1348AP

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA usingp-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at room temperature.
  A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
• Host: Donkey
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Mouse IgG and Mouse Serum.
  No reaction was observed against Bovine, Chicken, Donkey, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG snd protease free) as stabilizer.
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody (undiluted) at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial
  loss of enzymatic activity.
  Dilute only prior to immediate use.