Mouse IgG (H+L chain), adsorbed Donkey Polyclonal Antibody

CAT#: R1348P

Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  ELISA : 1:20,000 - 1:100,000
  Western Blot : 1:2,000 - 1:10,000
  Immunohistochemistry: 1:1,000 - 1:5,000
• Reactivities: Mouse
• Host: Donkey
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Mouse IgG and Mouse Serum.
  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) Ig fraction.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.