Mouse IgA (H+L chain) Goat Polyclonal Antibody

CAT#: R1349AP

Mouse IgA (H+L chain) goat polyclonal antibody, AP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/2,000-1/10,000.
  Western blot: 1/500-1/2,500.
  Immunohistochemistry: 1/200-1/1,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG, IgA and IgM whole molecules.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by mmunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Goat Serum.
  This reagent is suitable for the detection of all Mouse immunoglobulin isotypes, subclasses and chain combinations.
• Formulation: 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0, with 10 mg/ml BSA (IgG and protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative
  Label: AP
  State: Liquid (sterile filtered) purified Ig fraction.
  Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: AP
• Storage: Store the antibody (undiluted) at 2-8°C.
  DO NOT FREEZE!
  Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
  Dilute only prior to immediate use.