Mouse IgA (H+L chain) Goat Polyclonal Antibody

CAT#: R1349F

Mouse IgA (H+L chain) goat polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended dilutions:
  FLISA: 1/10,000-1/50,000
  Flow Cytometry: 1/500-1/2,500.
  IF Microscopy: 1/1,000-1/5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Mouse IgG, IgA and IgM wohle molecule.
• Specificity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM.
  This reagent is suitable for the detection of all mouse isotypes and chain combinations.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 2.9 moles FITC per mole of Goat IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280nm)
• Purification: Immunoaffinity Chromatography.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. For extended storage aliquot contents and freeze at -20°C or below.
  Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.