Mouse IgA (+IgG +IgM) Goat Polyclonal Antibody
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunoblotting (1/500-1/3,000 for Western blot), ELISA (1/100,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/250-1/1,500) as well as other Peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Mouse IgG, IgA and IgM whole molecules. |
Specificity | This product was prepared from polyspecific antiserum by Immunoaffinity Chromatography using antigens coupled to agarose beads. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all mouse immunoglobulin isotypes and chain combinations. |
Formulation | 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Thimerosal as preservative. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase Conjugation Chemistry: periodate |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Concentration | 2.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity Chromatography. |
Conjugation | HRP |
Storage | Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing. |
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