Mouse IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

CAT#: R1350HRP

Mouse IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (1/500-1/2,500, Western or dot blot), ELISA (1/5,000-1/20,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/200-1/1,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, and Mouse Serum.
  No reaction was observed against Human Serum Proteins.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin sulfate as preservative.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.