Mouse IgG (Fc specific) Rabbit Polyclonal Antibody

CAT#: R1352HRP

Mouse IgG (Fc specific) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (1/1,000-1/10,000), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry (1/500-1/2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western Blot : 1/1,000-1/10,000.
  Immunohistochemistry: 1/500-1/2,500.
  Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/50,000 of the reconstitution concentration is suggested for this product.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG F(c) fragment.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum.
  No reaction was observed against Mouse IgG F(ab')2.
• Formulation: 0.02M Potassium Phosphate, 0.14M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified IgG fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent)
  
  
• Concentration: 1.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Mouse IgG coupled to agarose
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.