Mouse IgM (Mu chain specific) Rabbit Polyclonal Antibody

CAT#: R1355TR

Mouse IgM (Mu chain specific) rabbit polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgM mu heavy chain
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgM and Mouse serum. No reaction was observed against Mouse IgG or Mouse IgA.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% Sodium Azide as preservative and 10 mg/ml BSA as stabilizer, IgG and Protease free.
  Label: Texas Red
  State: Lyophilized IgG fraction
  Label: (TM)* Sulfonyl Chloride (Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 2.4 moles Texas Red per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 1.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using mouse IgM coupled to agarose beads followed by solid phase adsorptions to remove any unwanted reactivities.
• Conjugation: Texas Red
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20° C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.
• Note: * Texas Red is a registered trademark of Molecular Probes Inc.