Mouse Kappa Light Chain Rabbit Polyclonal Antibody

CAT#: R1357AP

Mouse Kappa Light Chain rabbit polyclonal antibody, AP


USD 355.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for immunoblotting (Western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions:
ELISA 1:1,000 - 1:2,000.
Western Blot 1:500 - 1:1,000.
Immunohistochemistry: 1:200 - 1:1,000.
Host Rabbit
Clonality Polyclonal
Immunogen Mouse κ (kappa light chain)
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), and anti-Rabbit Serum. Solid phase adsorption(s) against a panel of Mouse IgG lambda, IgM lambda and IgA lambda proteins was performed. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Formulation 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0 ,with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: AP
State: Liquid (sterile filtered) immunoglobulin fraction
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration 1.1 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography
Conjugation AP
Storage Store vial at 4°C before opening. This product is stable at 4°C as an undiluted liquid. Dilute only prior to immediate use.
DO NOT FREEZE. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.