Mouse lambda light chain Rabbit Polyclonal Antibody

CAT#: R1358HRP

Mouse lambda light chain rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/10,000 - 1/50,000 .
  Western Blot: 1/1,000 - 1/5,000.
  Immunohistochemistry on paraffin sections: 1/500 - 1/2,500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse lambda light chain
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti- Peroxidase and anti-Rabbit Serum.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative
  Label: HRP
  State: Lyophilized purified Ig fraction
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed.
• Conjugation: HRP
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.