Mouse IgG1 (gamma 1 chain specific) Rabbit Polyclonal Antibody

CAT#: R1359AP

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, AP


USD 355.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using p-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,500 to 1:14,000 of the reconstitution concentration is suggested for this product.
Host Rabbit
Clonality Polyclonal
Immunogen Mouse IgG1 heavy chain.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum.
Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes.
Formulation 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, containing 10 mg/ml Bovine Serum Albumin (BSA IgG and Protease free) and 0.01% (w/v) sodium azide as preservative.
Label: AP
State: Liquid (sterile filtered) purified IgG fraction.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography.
Conjugation AP
Storage Store the antibody (undiluted) at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial
loss of enzymatic activity.
Dilute only prior to immediate use.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.