Mouse IgG1 (gamma 1 chain specific) Rabbit Polyclonal Antibody

CAT#: R1359F

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended dilutions:
  Flow cytometry 1:2,000 - 1:10,000
  IF microscopy 1:500 - 1:2,500
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG1 γ1 chain
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized
  Label: Fluorescein Isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 2.9 moles FITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: FITC
• Storage: Store vial at 4°C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4°C as an undiluted liquid.
  For extended storage aliquot contents after restoration and freeze at -20°C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.