Mouse IgG1 (gamma 1 chain specific) Rabbit Polyclonal Antibody

CAT#: R1359HRP

Mouse IgG1 (gamma 1 chain specific) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Anti Mouse IgG1 secondary antibody conjugate is suitable for ELISA, Immunohistochemistry, Western blotting as well as other anti IgG1 antibody based assays.
  Recommended Dilutions:
  ELISA: 1/300,000.
  Western Blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/500-1/2,500.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG1 gamma 1 heavy chain
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
  Specificity was confirmed by ELISA against other Mouse or Human heavy chain isotypes.
  Cross reactivity with Mouse IgG2b and IgG3 is negligible (< 0.5%).
  Cross reactivity with Mouse IgG2a and IgM is minimal.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: HRP
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.