Mouse IgG2a (gamma 2a chain specific) Rabbit Polyclonal Antibody

CAT#: R1360F

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, FITC

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution: This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  Immunofluorescence: 1/1,000-1/5,000.
  Flow Cytometry: 1/500-1/2,500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG2a heavy chain.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) against a panel of imobilized mouse and human serum proteins resulting in a conjugated antibody designed for Flow cytometry.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other Mouse or Human heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) as stabilzer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: F/P ratio: 4.7
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.