Mouse IgG2a (gamma 2a chain specific) Rabbit Polyclonal Antibody

CAT#: R1360HRP

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: This secondary antibody anti-Mouse is ideal for investigators who routinely perform titration assays, Western-blot, Immunoprecipitation and more generally immunoassays.
  Anti-Mouse IgG2a heavy chain antibody is suitable for highly specific immunological methods requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/90,000
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/500-1/2,500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG2a heavy chain
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity Chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
  Specificity was confirmed by ELISA at less than 1% cross reactivity against other Mouse or Human heavy or light chain isotypes.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: HRP
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.