Mouse IgG2a (gamma 2a chain specific) Rabbit Polyclonal Antibody

CAT#: R1360P

Mouse IgG2a (gamma 2a chain specific) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: This antibody is suitable for immunoprecipitation, Immunodiffusion, ELISA (1/40,000-1/200,000), Western blot (1/4,000-1/20,000), Immunohistochemistry (1/2,000-1/10,000), Immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG2a γ2a chain
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
  Specificty was confirmed by ELISA.
  Typically less than 1% cross reactivity was observed against other Mouse heavy chain isotypes.
• Formulation: 0.2 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilzer, 0.01% (w/v) Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid (sterile filtered) purified IgG fraction
• Concentration: 1.37 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.