Mouse IgG3 (gamma 3 chain specific) Rabbit Polyclonal Antibody

CAT#: R1362P

Mouse IgG3 (gamma 3 chain specific) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1/10,000-1/50,000.
  Western Blot: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Mouse IgG3 γ3 chain.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG.
  Maximum Cross Reactivity:
  X Reactivity: Limit (Actual)
  Mouse IgG1: < /= 2% (1.0%)
  Mouse IgG2a: < /= 2% (1.0%)
  Mouse IgG2b: < /= 2% (1.0%)
  Mouse IgG3: 100% (100%)
  Mouse IgM: < /= 10% (7.7%)
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) purified Ig fraction.
• Concentration: 1.15 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.