Rabbit IgG (H+L chain) Chicken Polyclonal Antibody

CAT#: R1363F

Rabbit IgG (H+L chain) chicken polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based Fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  IF Microscopy: 1/500-1/2,500.
• Host: Chicken
• Clonality: Polyclonal
• Immunogen: Rabbit IgG whole molecule.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Chicken Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Label: Highly purified isomer I of Fluorescein isothiocyanate. Care is taken to ensure complete removal of any free Fluorescein from the final product
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.8 moles FITC per mole of Chicken IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Prepared from monospecific antiserum by Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  This product is photosensitive and should be protected from light.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.