Rabbit IgG (H+L chain) Goat Polyclonal Antibody

CAT#: R1364F

Rabbit IgG (H+L chain) goat polyclonal antibody, FITC, Lyophilized

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: This product is designed for Immunofluorescence Microscopy, Fluorescence based plate assays (FLISA) and Fluorescent Western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  Immunofluoresence: 1/1,000-1/5,000.
  Fluorescent Western Blot.
• Reactivities: Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG, whole molecule.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: FITC
  State: Lyophilized
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (Ig and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.7 moles FITC per mole of Goat IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 2.0 mg/ml (after reconstitution) by UV absorbance at 280 nm
• Purification: Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after restoration and standing at RT.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
  Protect from light.