Rabbit IgG (H+L chain) Goat Polyclonal Antibody

CAT#: R1364HRP

Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized

Product Images

Western Blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody: Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH/3T3 Whole Cell Lysate. Load: 10 µg per lane. Primary antibody: Beta Actin Antibody at 1/1000 for overnight at 4°C. Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT . Predicted/Observed size: 42 kDa.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/150,000-1/250,000.
  Immunohistochemistry: 1/500-1/2,500.
  Western blot: 1/10,000-1/40,000.
• Reactivities: Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG, whole molecule
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (Ig and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
  Conjugation Chemistry: Periodate
  Label M/W: 40,000
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads
• Conjugation: HRP
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.