Rabbit IgG (H+L chain) Goat Polyclonal Antibody

CAT#: R1364T

Rabbit IgG (H+L chain) goat polyclonal antibody, TRITC, Lyophilized

Specifications

Product Data

• Applications: ELISA, FC, IF
• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1,000-1/5,000.
• Reactivities: Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG, whole molecule
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: TRITC
  State: Lyophilized
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 2.5 moles TRITC per mole of Goat IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent)
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm before lyophil.)
• Purification: Immunoaffinity Chromatography using Rabbit IgG coupled to agarose.
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at RT.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.