Rabbit IgG (H+L chain) Goat Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, FC, IF |
Recommended Dilution | Suitable for immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1000 - 1/5000. |
Reactivities | Rabbit |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Rabbit IgG whole molecule |
Specificity | Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit serum. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Label: Texas Red State: Lyophilized State: Lyophilized IgG fraction Stabilizer: 10 mg/ml BSA (IgG and Protease free) Preservative: 0.01% Sodium Azide Label: (TM)* Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption emission: 596 nm / 620 nm Molar radio: 4.5 moles Texas Red per mole of Goat IgG |
Reconstitution Method | Restore with 2.0 ml of deionized water or equivalent. |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography using rabbit IgG coupled to agarose |
Conjugation | Texas Red |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Note | * Texas Red is a registered trademark of Molecular Probes Inc. |
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