Rabbit IgG (H+L chain, adsorbed) Goat Polyclonal Antibody

CAT#: R1365HRP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/10,000-1/150,000.
  Western blot: 1/1,500-1/5,000.
  Immunohistochemistry: 1/750-1/2,500.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG, whole molecule.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum.
  No reaction was observed against Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free)as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
  Label: HRP
  State: Lyophilized purified fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  For extended storage aliquot contents after reconstitution and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.
• Note: Do Not add Sodium Azide!
  Minimum Cross Reactivity to Human Serum Proteins.