Rabbit IgG (Fc specific) Goat Polyclonal Antibody

CAT#: R1366F

Rabbit IgG (Fc specific) goat polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  FLISA: 1/10,000-1/50,000
  IF: 1/1,000-1/5,000
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG F(c) fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab')2.
• Formulation: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA; IgG and Protease free) as stabilizer and 0.01% (w/v) Thimerisol as preservative.
  Label: FITC
  State: Lyophilised purified IgG
  Label: Diaminotriazinylaminofluorescein (DTAF) Molecular Weight 530 daltons; is in all aspects equivalent to
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.5 moles DTAF per mole of Goat IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.