Rabbit IgG (Fc specific) Goat Polyclonal Antibody

CAT#: R1366HRP

Rabbit IgG (Fc specific) goat polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/110,000.
  Western Blot: 1/2,000-1/20,000.
  Immunohistochemistry: 1/1,000-1/5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rabbit IgG F(c) fragment.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum.
  No reaction was observed against Rabbit IgG F(ab')2.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Gentamicin Sulfate as preservative and 10 mg/ml BSA (IgG and Protease free) as stabilizer.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Rabbit IgG coupled to agarose beads.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration. Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature.
  This product is stable for several weeks at 2-8° C as an undiluted liquid.
  Dilute only prior to immediate use.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.