Rat IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1371B

Rat IgG (H+L chain) rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/500,000.
  Western blot: 1/2,000-1/10,000.
  Immunohistochemistry: 1/1,000-1/5,000.
  Note: When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat IgG whole molecule.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Rat IgG and Rat Serum.
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2
  Label: Biotin
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% Sodium Azide
  Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
  Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: Biotin
• Storage: Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 2-8°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.