Rat IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

CAT#: R1372HRP

Rat IgG (H+L chain), adsorbed rabbit polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/2,000-1/10,000.
  Western Blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat IgG whole molecule.
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Rat IgG and Rat Serum.
  No reaction was observed against Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
  Label: HRP
  State: Lyophilized purified Ig fraction.
  Label: Horseradish Peroxidase Conjugation Chemistry: Periodate. Label MW: 40,000
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation: HRP
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature.
  This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
  Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.