Rat IgM (Mu chain specific) Rabbit Polyclonal Antibody

CAT#: R1375B

Rat IgM (Mu chain specific) rabbit polyclonal antibody, Biotin


USD 305.00

3 Days*

Size
    • 1.5 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 ug of Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/15,000 to 1/70,000 of the concentration is suggested for this product.
Host Rabbit
Clonality Polyclonal
Immunogen Rat IgM µ chain.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Rat IgM and Rat Serum.
No reaction was observed against Rat IgA or Rat IgG.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
Label: Biotin
State: Lyophilized purified Ig fraction.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Rabbit IgG molecule.
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration 1.5 mg/ml (prior to lyophil., by UV absorbance at 280 nm)
Purification Immunoaffinity Chromatography.
Conjugation Biotin
Storage Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 2-8°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid repeated freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.