Rat IgM (Mu chain specific) Rabbit Polyclonal Antibody

CAT#: R1375P

Rat IgM (Mu chain specific) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  ELISA: 1:50,000-1:250,000.
  Western Blot: 1:5,000-1:25,000.
  Immunohistochemistry: 1:1,000-1:5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Rat IgM mu heavy chain.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgM coupled to agarose followed by solid phase adsorption(s).
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgM and Rat Serum.
  No reaction was observed against other Rat or human heavy or light chain proteins.
  Specificity was confirmed by ELISA at less than 0.4% cross reactivity against Rat IgG and Rat IgA.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid (sterile filtered) purified Ig fraction.
• Concentration: 2.4 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Storage: Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.