Rat IgA (alpha chain) Goat Polyclonal Antibody
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1:10,000-1:50,000. Western Blot: 1:1,000-1:5,000. Immunohistochemistry: 1:500-1:3,000. |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Rat IgA α chain |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against Rat IgM or Rat IgG. Specificity was confirmed by ELISA at less than 1% cross reactivity against other Rat heavy or light chain isotypes. |
Formulation | 0.1 M Sodium Borate, 0.005 M EDTA, 0.25 M Sodium Chloride, pH 8.2 containing 0.01% (w/v) Sodium Azide as preservative. State: Aff - Purified State: Liquid (sterile filtered) purified Ig fraction |
Concentration | 1.0 mg/ml (by UV absorbance at 280 nm) |
Purification | Immunoaffinity chromatography |
Storage | Store the antibody (undiluted) at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. |
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