Rat IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody

CAT#: R1377F

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  Flow Cytometry: 1/2,000-1/10,000.
  Immunofluorescence microscopy: 1/1,000-1/5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rat IgG, IgA and IgM whole molecules.
• Specificity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM.
  This reagent is suitable for the detection of all Rat isotypes and chain combinations.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: FITC
  State: Lyophilized purified Ig fraction.
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Sodium Azide
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 3.5 moles FITC per mole of Goat IgG.
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: FITC
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.