Rat IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody

CAT#: R1377TR

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Texas Red

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1:2,000-1:10,000.
  Western Blot: 1:500-1:2,500.
  Immunohistochemistry: 1:200-1:1,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Rat IgG, IgA and IgM whole molecules.
• Specificity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.
  This product is suitable for the detection of all Rat immunoglobulin classes, isotypes and chain combinations.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01% (w/v) Sodium Azide as preservative.
  Label: Texas Red
  State: Lyophilized purified Ig fraction.
  Label: -- Sulfonyl Chloride (TR; Molecular Weight 625 daltons)
  Absorption emission: 596 nm / 620 nm
  Molar radio: 2.9 moles Texas Red￤ per mole of Goat IgG.
• Reconstitution Method: Restore with 0.5 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography.
• Conjugation: Texas Red
• Storage: Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 4°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.