Sheep IgG (H+L chain), adsorbed Rabbit Polyclonal Antibody

CAT#: R1379F

Sheep IgG (H+L chain), adsorbed rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG and Sheep Serum. No reaction was observed against Human Serum Proteins.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide as preservative
  Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free
  Label: FITC
  State: Lyophilized Ig fraction
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495nm / 528nm
  Molar radio: 4.9 moles FITC per mole of Rabbit IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (by Uv absorbance at 280nm)
• Purification: Immunoaffinity chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8° C prior to restoration. Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid.
  For extended storage add glycerol to 50% and then aliquot contents and freeze at -20° C or below.
  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.