Sheep IgG F(ab)2 specific Rabbit Polyclonal Antibody

CAT#: R1382AP

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, AP


USD 325.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions: This product has been assayed against 1.0 µg of Sheep IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product.
Host Rabbit
Clonality Polyclonal
Immunogen Sheep IgG F(ab')2 fragment.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab') 2 and Sheep Serum.
No reaction was observed against Sheep IgG F(c).
Formulation 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free as stabilizer and 0.01% (w/v) sodium azide as preservative.
Label: AP
State: Liquid (sterile filtered) purified IgG fraction.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Concentration 0.6 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography.
Conjugation AP
Storage Store the antibody (undiluted) at 2-8°C.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial
loss of enzymatic activity.
Dilute only prior to immediate use.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.