Sheep IgG F(ab)2 specific Rabbit Polyclonal Antibody

CAT#: R1382F

Sheep IgG F(ab)2 specific rabbit polyclonal antibody, FITC

Specifications

Product Data

• Recommended Dilution: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. It is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
  FLISA: 1/10,000-1/50,000.
  IF Microscopy: 1/1,000-1/5,000.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Sheep IgG F(ab')2 fragment.
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(ab')2 and Sheep Serum.
  No reaction was observed against Sheep IgG F(c).
• Formulation: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: FITC
  State: Lyophilized purified Ig fraction
  Stabilizer: 0.01% (w/v) Sodium Azide
  Preservative: 10 mg/ml BSA
  Label: Isothiocyanate
  Label MW: 390
  Absorption emission: 495 nm / 528 nm
  Molar radio: F/P Ratio = 2.8
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.5 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: FITC
• Storage: Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below.
  Centrifuge product if not completely clear after standing at room temperature.
  This antibody is stable for one month at 4°C as an undiluted liquid.
  Dilute only prior to immediate use.
  Avoid cycles of freezing and thawing.