Porcine IgG (H+L chain) Rabbit Polyclonal Antibody

CAT#: R1384HRP

Porcine IgG (H+L chain) rabbit polyclonal antibody, HRP

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Recommended Dilutions:
  ELISA: 1/150,000.
  Western blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,500.
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Swine IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG and Swine Serum.
• Formulation: 0.02 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
  Label: HRP
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (IgG and Protease free)
  Preservative: 0.01% (w/v) Gentamicin Sulfate
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 2.0 mg/ml (OD 280 nm)
• Purification: Immunoaffinity Chromatography
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.