Porcine IgG F(c) specific Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Recommended Dilution | Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommendeed Dilutions: This product has been assayed against 1.0 µg of Swine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:6,500 to 1:27,000 of the reconstitution concentration is suggested for this product. |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Swine IgG F(c) fragment. |
Specificity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG, Swine IgG F(c) and Swine Serum. No reaction was observed against Swine IgG F(ab')2. |
Formulation | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% (w/v) Gentamicin sulfate as preservative and 10 mg/ml Bovine Serum Albumin (BSA; IgG and Protease free) as stabilizer. Label: HRP State: Lyophilized purified Ig fraction. Label: Horseradish Peroxidase |
Reconstitution Method | Restore with 1.0 ml of deionized water (or equivalent). |
Purification | Immunoaffinity chromatography. |
Conjugation | HRP |
Storage | Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. |
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