Porcine IgG F(ab)2 specific Rabbit Polyclonal Antibody

CAT#: R1386AP

Porcine IgG F(ab)2 specific rabbit polyclonal antibody, AP


USD 330.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1:1,000-1:3,000.
Western Blot: 1:500-1:2,000.
Immunohistochemistry: 1:200-1:1,000.
Host Rabbit
Clonality Polyclonal
Immunogen Swine IgG F(ab')2 fragment.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Swine IgG, Swine IgG F(ab') 2 and Swine Serum.
No reaction was observed against Swine IgG F(c).
Formulation 0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0 with 10 mg/ml Bovine Serum Albumin (BSA) IgG
Label: AP
State: Lyophilized purified Ig fraction.
Label: Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Reconstitution Method Restore with 0.5 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography.
Conjugation AP
Storage Store the antibody (undiluted) at 2-8°C before opening.
DO NOT FREEZE!
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Dilute only prior to immediate use.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.