Human IgG (H+L chain), Fab Fragment Goat Polyclonal Antibody

CAT#: R1388F

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, FITC

Product Images

Western Blot of Goat anti-Fab Human IgG (H&L) Antibody Fluorescein Conjugated: Lane 1: Human IgG Load: 50 ng per lane Secondary antibody: Fab Human IgG (H&L) Antibody Fluorescein Conjugated at 1/1,000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa

Specifications

Product Data

• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
  Recommended Dilutions:
  FLISA: 1/10,000-1/50,000.
  Flow Cytometry: 1/500-1/2,500.
  IF Microscopy: 1/1,000-1/5,000.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum.
  No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Formulation: 0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA (IgG and Protease free) and 0.01% (w/v) Sodium Azide
  Label: FITC
  State: Lyophilized Fab fragment
  Label: Fluorescein isothiocyanate (Molecular Weight 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 4.1 moles FITC per mole of Goat IgG Fab
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Concentration: 1.0 mg/ml (UV absorbance at 280 nm)
• Purification: Immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.