Human IgG (H+L chain), Fab Fragment Goat Polyclonal Antibody

CAT#: R1388HRP

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, HRP

Specifications

Product Data

• Recommended Dilution: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
  Note: This product has been assayed against 1.0 µg of Human IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
  Recommended Dilutions:
  ELISA: 1/300,000
  Western blot: 1/1,000-1/5,000.
  Immunohistochemistry: 1/500-1/2,500.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule.
• Specificity: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum.
  No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA (IgG and Protease free) as stabilizer and 0.01% (w/v) Gentamicin Sulfate as preservative.
  Label: HRP
  State: Lyophilized Fab fragments.
  Label: Horseradish Peroxidase
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)
• Purification: Immunoaffinity Chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
• Conjugation: HRP
• Storage: Store vial at 4°C prior to restoration.
  Restore with deionized water or equivalent; centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 4°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below.
  Avoid cycles of freezing and thawing.
  Dilute only prior to immediate use.