Human IgG (H+L chain), Fab Fragment Goat Polyclonal Antibody

CAT#: R1388T

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, TRITC

Specifications

Product Data

• Recommended Dilution: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Human IgG whole molecule
• Specificity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 10 mg/ml BSA, IgG and Protease free, and 0.01% (w/v) Sodium Azide
  Label: TRITC
  State: Lyophilized Fab fragments
  Label: Tetramethylrhodamine isothiocyanante (Molecular Weight 444 daltons)
  Absorption emission: 550 nm / 570 nm
  Molar radio: 1.2 moles TRITC per mole of goat IgG Fab
• Reconstitution Method: Restore with 1.0 ml of deionized water or equivalent.
• Purification: Immunoaffinity chromatography
• Conjugation: TRITC
• Storage: Store vial at 2-8°C prior to restoration.
  Restore with deionized water (or equivalent); centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid.
  For extended storage aliquot contents and freeze at -20°C or below. Avoid repeated freezing and thawing.
  Dilute only prior to immediate use.