Rabbit IgG (H+L chain) (Fab Fragment) Goat Polyclonal Antibody

CAT#: R1390B

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, Biotin


USD 280.00

3 Days*

Size
    • 1 mg

Product Images

Specifications

Product Data
Recommended Dilution Suitable for Western Blot, ELISA,
Immunohistochemistry, Immunomicroscopy, as well as other antibody based assays using streptavidin or avidin conjugates requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:250,000 of the reconstitution concentration is suggested for this product.
Host Goat
Clonality Polyclonal
Immunogen Rabbit IgG whole molecule.
Specificity This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Assay by immuno-electrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum and Rabbit IgG.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c).
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01% (w/v) Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
Label: Biotin
State: Lyophilized Fab fragments.
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Goat IgG molecule.
Reconstitution Method Restore with 1.0 ml of deionized water (or equivalent).
Concentration 1.0 mg/ml (by UV absorbance at 280 nm)
Purification Immunoaffinity chromatography.
Conjugation Biotin
Storage Store vial at 4°C prior to restoration. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20°C or below.
Centrifuge product if not completely clear after standing at room temperature.
This antibody is stable for one month at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Avoid cycles of freezing and thawing.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.